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Anti-Hu CD15 Purified

CD15 (Lewis x), also known as stage specific embryonic antigen-1 (SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several structural relatives of Lewis x, e.g. sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le (x) antigen exhibit strong self-aggregation, based on calcium-dependent Le (x) -Le (x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le (x) and its isomer sialyl-Le (a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

WERI-RB-1 retinoblastoma cell line

Target Antigen

CD15

Clone

W6D3

Applications

FC (QC tested)

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Mandalasi M, Dorabawila N, Smith DF, Heimburg-Molinaro J, Cummings RD, Nyame AK: Development and characterization of a specific IgG monoclonal antibody toward the Lewis x antigen using splenocytes of Schistosoma mansoni-infected mice. Glycobiology. 2013 Jul;23 (7) :877-92., URL: https://www.ncbi.nlm.nih.gov/pubmed/23542315, *Sutherland DR, Ortiz F, Quest G, Illingworth A, Benko M, Nayyar R, Marinov I: High-sensitivity 5-, 6-, and 7-color PNH WBC assays for both Canto II and Navios platforms. Cytometry B Clin Cytom. 2018 Jul;94 (4) :637-651., URL: https://www.ncbi.nlm.nih.gov/pubmed/29381839, *Brazil JC, Sumagin R, Cummings RD, Louis NA: Targeting of neutrophil Lewis X blocks transepithelial migration and increases phagocytosis and degranulation. Am J Pathol. 2016 Feb;186 (2) :297-311., URL: https://pubmed.ncbi.nlm.nih.gov/26687991/, *Carrel J, Groves CJ: OMIP-043: Identification of human antibody secreting cell subsets. Cytometry A. 2018 Feb;93 (2) :190-193., URL: https://pubmed.ncbi.nlm.nih.gov/29286577/

Other References

*Benharroch D, Dima E, Levy A, Ohana-Malka O, Ariad S, Prinsloo I, Mejirovsky E, Sacks M, Gopas J: Differential expression of sialyl and non-sialyl-CD15 antigens on Hodgkin-Reed-Sternberg cells: significance in Hodgkin's disease. Leuk Lymphoma. 2000 Sep;39 (1-2) :185-94., URL: https://pubmed.ncbi.nlm.nih.gov/10975398/, *Hakomori S: Le (X) and related structures as adhesion molecules. Histochem J. 1992 Nov;24 (11) :771-6., URL: https://pubmed.ncbi.nlm.nih.gov/1362193/, *Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le (X) but is inhibited by sLe (X) . Biochem J. 2007 Sep 1;406 (2) :333-41., URL: https://pubmed.ncbi.nlm.nih.gov/17497959/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

Mouse monoclonal antibody W6D3 recognizes CD15 in nonterminal position on extracellular glycoproteins. CD15 (a cell membrane 3-fucosyl-N-acetyllactosamine; 3-FAL) is strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. This is a non-IgM anti-CD15 antibody.

Applications Notes

Flow cytometry: Recommended dilution: 1-4 µg/ml

Isotype

Mouse IgG1 kappa

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