Anti-Hu SIGLEC8 Purified

SIGLEC8 is a sialic acid binding lectin similar to CD33. In its cytoplasmic comain it contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), and a motive similar to a binding site for SLAM-associated protein. SIGLEC8 is expressed e.g. in lymph nodes and spleen. It is an eosinophil marker, although it can be found also on the surface of mast cells. Crosslinking of SIGLEC8 leads to apoptosis. Soluble form of SIGLEC8 can be foud in human serum, especially in case of eosinophil-associated diseases.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Extracellular domain of human SIGLEC8 fused with Fc fragment of human IgG1

Target Antigen

SIGLEC8

Clone

7C9

Applications

FC (QC tested), ELISA, IP

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Galeotti C, Stephen-Victor E, Karnam A, Das M, Gilardin L, Maddur MS, Wymann S, Vonarburg C, Chevailler A, Dimitrov JD, Benveniste O, Bruhns P, Kaveri SV, Bayry J: Intravenous immunoglobulin induces IL-4 in human basophils by signaling through surface-bound IgE. J Allergy Clin Immunol. 2019 Aug;144 (2) :524-535.e8., URL: https://www.ncbi.nlm.nih.gov/pubmed/30529242, *Kano G, Bochner BS, Zimmermann N: Regulation of Siglec-8-induced intracellular reactive oxygen species production and eosinophil cell death by Src family kinases. Immunobiology. 2017 Feb;222 (2) :343-349., URL: https://www.ncbi.nlm.nih.gov/pubmed/27682013, *Arakawa S, Suzukawa M, Ohshima N, Tashimo H, Asari I, Matsui H, Kobayashi N, Shoji S, Nagase T, Ohta K: Expression of Siglec-8 is regulated by interleukin-5, and serum levels of soluble Siglec-8 may predict responsiveness of severe eosinophilic asthma to mepolizumab. Allergol Int. 2018 Sep;67S:S41-S44., URL: https://www.ncbi.nlm.nih.gov/pubmed/29703694, *Kano G, Almanan M, Bochner BS, Zimmermann N: Mechanism of Siglec-8-mediated cell death in IL-5-activated eosinophils: role for reactive oxygen species-enhanced MEK/ERK activation. J Allergy Clin Immunol. 2013 Aug;132 (2) :437-45., URL: https://pubmed.ncbi.nlm.nih.gov/23684072/