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Anti-Dendra2 purified

Dendra2 is an improved version of green-to-red photoswitchable protein Dendra, and compared to it, Dendra2 exhibits brighter fluorescence before and after photoswitching. Excitation maximum of Dendra2 is 490 nm before and 553 nm after photoactivation, and its emission maximum is 507 nm before and 573 nm after photoactivation. Activating light for Dendra2 is UV/violet to blue. Nonactivated Dendra2 spectral characteristics are similar to EGFP, and this green fluorescence can be detected at low light intensities of blue light. At hight intensities of the same blue light (or of UV/violet light) Dendra2 is photoactivated and gets emission characteristics similar to TRITC.

Product Specifications

Certification

RUO

Immunogen

Dendra2 tag

Target Antigen

Dendra2

Clone

PAb (836)

Applications

WB, ICC, FC (QC tested)

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Patrizio A, Renner M, Pizzarelli R, Triller A, Specht CG: Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers. Sci Rep. 2017 Sep 7;7 (1) :10899., URL: https://pubmed.ncbi.nlm.nih.gov/28883437/, *McEwen AE, Maher MT, Mo R, Gottardi CJ: E-cadherin phosphorylation occurs during its biosynthesis to promote its cell surface stability and adhesion. Mol Biol Cell. 2014 Aug 15;25 (16) :2365-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/24966173

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The rabbit polyclonal antibody PAb (836) recognizes Dendra2 tag, a green-to red photoswitchable protein derived from an octocoral (Dendronephthya) .

Species

Species independent

CAS Number

9007-83-4

Isotype

Rabbit polyclonal

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