CTCF Antibody - N-terminal region
Product Specifications
CAS Number
9007-83-4
Gene Name
CCCTC-binding factor (zinc finger protein)
Gene Aliases
MRD21, FAP108, CFAP108
Gene ID
10664
Accession Number
NP_006556
Reactivity
Human, Mouse, Rat, Cow, Dog, Horse, Rabbit
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human CTCF
Target
CTCF is a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT) -containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC) -containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in CTCF have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT) -containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC) -containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.
Partner Proteins
ZMYM4; UBC; EED; BMI1; KANSL1; CDK19; ZMYM2; CAND1; ZBTB33; TFAP4; SUMO1; SUMO2; SUMO3; SIRT7; TAF3; SMC3; POLR2A; SUZ12; Ctcf; HIST2H2AC; TOP2A; TAF1; NPM1; LMNA; KPNA4; KPNA2; H2AFZ; PARP1; TRB; RXRA; SMAD3; SIN3A; SMC1A; RAD21; YY1; THRB; SMAD4; HMGB1
Clonality
Polyclonal
Type
Polyclonal Antibody
Sequence
GELPPQEDPSWQKDPDYQPPAKKTKKTKKSKLRYTEEGKDVDVSVYDFEE
Applications
CHIP, WB
Purification
Affinity Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.5 mg/ml
Homology
Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Molecular Weight
83kDa
Protein Length
727
NCBI Gene Symbol
CTCF
Host or Source
Rabbit
Protein Name
Transcriptional repressor CTCF
Gene Name URL
CTCF
Nucleotide Accession Number
NM_006565
Curated Selection
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