Human IgM (myeloma) Fab u fragment Biotin conjugated Protein
Product Specifications
Gene Aliases
Human IgM Fab μ fragment Biotin conjugated, Human myeloma IgM Fabμ fragment Biotin conjugation
Reactivity
Human
Target
Human IgM Fab mu Biotin is ideal for investigators involved in serum protein component research. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to antigen. The spleen is the major site of specific IgM production. Distinct heavy chains differ in size and composition; alpha and gamma contain approximately 450 amino acids, while and have approximately 550 amino acids.
Conjugation
Biotin
Type
Protein
Purification
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by enzyme digestion and extensive dialysis against the buffer stated above.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Lyophilized
Reconstitution
2°C to 8°C|-20°C or -80°C
Molecular Weight
455
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Human IgM, anti-Human IgG F (ab’) 2 and anti-Human Serum. No reaction was observed against anti-Human IgM Fc5u.
Protein Length
Native
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