IgG1 subclass Fluorescein conjugated Protein

Product Specifications

Reactivity

Mouse

Clonality

Monoclonal

Conjugation

FITC

Type

Protein

Applications

Enzyme-linked immunosorbent assay|Flow cytometry|Fluorescence-linked immunosorbent assay|Immunofluorescence|Western blot

Purification

This product has been prepared from normal mouse serum by immunoaffinity chromatography using antibodies coupled to agarose beads. Extensive cross-adsorbtion was performed to remove any unwanted subclasses. In_x005F an Ouchterlony double diffusion assay, the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Lyophilized

Reconstitution

2°C to 8°C|-20°C or -80°C

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Typically less than 1% cross reactivity against other mouse heavy or light chains isotypes was detected by ELISA.