Recombinant Streptavidin Protein

Product Specifications

CAS Number

9000-83-3

Gene Aliases

Streptavidin, Streptavidin Recombinant

Target

Streptavidin Streptomyces Avidinii Recombinant produced in E.Coli. Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is widely used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14) . The complexes are also extremely stable over a wide range of temperature and pH.

Type

Protein

Source

E. coli

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml (prior to lyophilization)

Bioactivity

Specific Activity: //> 17 U/mg (one unit binds 1 ug D-biotin at pH 8.0) . _x005F Proteolytic Activity: //< 10-3 U/mg protein (Azocoll, 25°C, 24 h, pH 8.0) .Proteolytic Activity: //< 10-3 U/mg protein (Azocoll, 25°C, 24 h, pH 8.0) .-3

Format

Lyophilized//in 10mM potassium phosphate buffer pH 6.5 Physical Appearance: //Sterile filtered white powder.Physical Appearance: //Sterile filtered white powder.