Leptin qA Protein

Product Specifications

Gene Aliases

Leptin qA Human, Leptin Antagonist Quadruple Mutant Human Recombinant

Reactivity

Human

Target

Recombinant Human Leptin Quadruple Antagonist

Type

Protein

Sequence

The sequence of the first five N - terminal amino acids was determined and was found to be Ala - Val - Pro - Ile - Gln.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Format

The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. Physical appearance: White lyophilized (freeze-dried) powder.

Reconstitution

Lyophilized Leptin Antagonist Quadruple Mutant although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0. 1 Leptin mutant mg/ml and up to 2 mM and filter sterilization LEP mutant can be stored at 4C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0. 1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.