DnaK Recombinant Protein (E.Coli)
Product Specifications
CAS Number
9000-83-3
Gene Name
Chaperone protein DnaK
Gene Aliases
B0014; chaperone protein DnaK; ECK0014; groPAB; groPC; groPF; grpC; grpF; Heat shock 70 kDa protein; Heat shock protein 70; HSP70; seg.
Gene ID
944750
Accession Number
NP_001138811.1
Reactivity
Escherichia coli
Target
Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock.
Type
Protein
Applications
ELISA, WB
Purification
Purified by multi-step chromatography.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Format
Liquid. In 40mM TRIS, pH 7.5, containing 80mM sodium chloride, 0.8mM DTT, 0.08mM PMSF, and 20% glycerol.
Reconstitution
Store at -80C
Molecular Weight
70 kDa
Protein Length
Recombinant
NCBI Gene Symbol
DnaK
Host or Source
E. Coli
Protein Name
Chaperone protein DnaK
Gene Name URL
DnaK
Nucleotide Accession Number
NM_001145339.2
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