Collagen I, rat tail (Rat)
Product Specifications
Accession Number
NP_002147.2
Reactivity
Rat
Target
Collagen is the main component in connective tissue and helps to provide support for tissues. It is made up of several classes, with Type 1 collagen being the most common. Type 1 collagen has a herterotrimeric triple helical structure made up of two alpha-1 (I) and one alpha-2 (I) chains that form into elongated fibrils which are extremely strong. These fibrils can be found in skin, tendons, ligaments, and other connective tissues. Type 1 collagen has been shown to be useful as a substrate that promotes cell growth and proliferation. Under acidic conditions the protein is soluble, however by raising the temperature and pH the solution forms into a solid gel that can be useful for cellular studies. It can also be dried to form a thin layer on solid surfaces such as plates, slides, or coverslips to aid in cell attachment.
Type
Protein
Source
Isolated from rat tail tendons.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
5 mg/ml
Bioactivity
Tested in cell proliferation assay -- Increased attachment of cells on collagen coated coverslips.
Format
Liquid// 0.02N acetic acid Physical Appearance: //Hazy viscous liquidPhysical Appearance: //Hazy viscous liquid
Reconstitution
Store at 4° C. Keep sterile. Do not freeze.
Molecular Weight
235 kDa, 215 kDa, 130 kDa, 115 kDa
NCBI Gene Symbol
COLLAGEN I
Host or Source
Rat
Protein Name
Collagen
Gene Name URL
COLLAGEN I
Nucleotide Accession Number
NM_002156.4
Frequently Asked Questions
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