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Collagen I, rat tail (Rat)

Product Specifications

Accession Number

NP_002147.2

Reactivity

Rat

Target

Collagen is the main component in connective tissue and helps to provide support for tissues. It is made up of several classes, with Type 1 collagen being the most common. Type 1 collagen has a herterotrimeric triple helical structure made up of two alpha-1 (I) and one alpha-2 (I) chains that form into elongated fibrils which are extremely strong. These fibrils can be found in skin, tendons, ligaments, and other connective tissues. Type 1 collagen has been shown to be useful as a substrate that promotes cell growth and proliferation. Under acidic conditions the protein is soluble, however by raising the temperature and pH the solution forms into a solid gel that can be useful for cellular studies. It can also be dried to form a thin layer on solid surfaces such as plates, slides, or coverslips to aid in cell attachment.

Type

Protein

Source

Isolated from rat tail tendons.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

5 mg/ml

Bioactivity

Tested in cell proliferation assay -- Increased attachment of cells on collagen coated coverslips.

Format

Liquid// 0.02N acetic acid Physical Appearance: //Hazy viscous liquidPhysical Appearance: //Hazy viscous liquid

Reconstitution

Store at 4° C. Keep sterile. Do not freeze.

Molecular Weight

235 kDa, 215 kDa, 130 kDa, 115 kDa

NCBI Gene Symbol

COLLAGEN I

Host or Source

Rat

Protein Name

Collagen

Gene Name URL

COLLAGEN I

Nucleotide Accession Number

NM_002156.4

Frequently Asked Questions

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