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CDC25B Recombinant Protein (Human)

Product Specifications

CAS Number

9000-83-3

Gene Name

Cell division cycle 25B

Gene Aliases

CDC25 homolog B; dual specificity phosphatase Cdc25B; M-phase inducer phosphatase 2.

Gene ID

994

Accession Number

NP_001017963.2

Reactivity

Homo sapiens|Human

Target

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

Type

Protein

Purification

Partially purified by single-step affinity chromatography and gel filtration.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Format

Liquid. In 50mM TRIS/HCl, pH 8.0, 150mM NaCl, 5mM DTT, 0.1mM EDTA, 0.03% Brij 35, 20% glycerol.

Reconstitution

Store at -80C

Molecular Weight

65 kDa

Protein Length

Recombinant

NCBI Gene Symbol

CDC25B

Host or Source

E. Coli

Protein Name

M-phase inducer phosphatase 2

Gene Name URL

CDC25B

Nucleotide Accession Number

NM_001017963.2

Frequently Asked Questions

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