TNPO1 Recombinant Protein
Product Specifications
CAS Number
9000-83-3
Gene Name
Transportin 1
Gene Aliases
AU021749; D13Ertd688; D13Ertd688e; importin beta-2; IPO2; karyopherin (importin) beta 2; karyopherin beta-2; Kpn; Kpnb2; MIP; MIP1; transportin-1; TRN.
Gene ID
238799
Accession Number
NP_001041732.1
Reactivity
Mouse|Mus musculus
Target
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed:11493596) . Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity) . Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A (By similarity) . In vitro, mediates nuclear import of SRP19 (By similarity) . Mediates the import of histones H2A, H2B, H3 and H4 (PubMed:11493596) . Mediates nuclear import of ADAR/ADAR1 in a RanGTP-dependent manner (By similarity) .
Conjugation
Unconjugated
Type
Protein
Source
E.coli
Applications
Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
200 ug/mL (prior to lyoph)
Format
Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Reconstitution
2°C to 8°C|-80°C
Molecular Weight
26kDa
Protein Length
Recombinant
NCBI Gene Symbol
Tnpo1
Host or Source
E.coli
Protein Name
Transportin-1
Gene Name URL
Tnpo1
Nucleotide Accession Number
NM_001048267.1
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
