Recombinant Human Melanoma Inhibitory Activity Protein
Product Specifications
Gene ID
8190
UniProt
Q16674
Target
GPMPKLADRK LCADQECSHP ISMAVALQDY MAPDCRFLTI HRGQVVYVFS KLKGRGRLFW GGSVQGDYYG DLAARLGYFP SSIVREDQTL KPGKVDVKTD KWDFYCQ
Purification
> 98 % by SDS-PAGE and HPLC analyses.
Endotoxin
Less than 0.1 EU/ug of rHuMIA as determined by LAL method.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human A375 cell line is less than 5 μg/ml, corresponding to a specific activity of > 200 IU/mg.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculated Molecular Weight
Approximately 12.1 kDa, a single non-glycosylated polypeptide chain containing 107 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % Trehalose.
Host or Source
Escherichia coli
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