PRKAG1 Recombinant Protein
Product Specifications
CAS Number
9000-83-3
Gene Name
Protein kinase AMP-activated non-catalytic subunit gamma 1
Gene Aliases
5'-AMP-activated protein kinase subunit gamma-1;5'-AMP-activated protein kinase, gamma-1 subunit; AMPK gamma1; AMPK gamma-1 chain; AMPKG; protein kinase, AMP-activated, gamma 1 non-catalytic subunit.
Gene ID
5571
Accession Number
NP_001193638.1
Reactivity
Homo sapiens|Human
Target
AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
Conjugation
Unconjugated
Type
Protein
Source
E.coli
Applications
Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
200 ug/mL (prior to lyoph)
Format
Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Reconstitution
2°C to 8°C|-80°C
Molecular Weight
30kDa
Protein Length
Recombinant
NCBI Gene Symbol
PRKAG1
Protein Name
5'-AMP-activated protein kinase subunit gamma-1
Gene Name URL
PRKAG1
Nucleotide Accession Number
NM_001206709.1
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
E2270-10E-APC 200 µL
EN2, CT (Homeobox Protein Engrailed-2, Homeobox Protein en-2, Hu-En-2) (APC) discontinued
Ask
View Details MBS7152926 Inquire
Rabbit anti-Escherichia phage HK022 (Bacteriophage HK022) kil Polyclonal Antibody
Ask
View Details 
abx507000 96 Tests
Human CKLF-like MARVEL transmembrane domain-containing protein 3 (CMTM3) ELISA Kit
Ask
View Details