PLEC Recombinant Protein

Product Specifications

CAS Number

9000-83-3

Gene Name

Plectin

Gene Aliases

EBS1; EBSMD; EBSND; EBSO; EBSOG; EBSPA; HD1; hemidesmosomal protein 1; LGMD2Q; LGMDR17; PCN; PLEC1; PLEC1b; plectin; plectin 1, intermediate filament binding protein 500kDa; Plectin-1; PLTN.

Gene ID

5339

Accession Number

NP_000436.2

Reactivity

Homo sapiens|Human

Target

Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofiber integrity.

Conjugation

Unconjugated

Type

Protein

Source

E.coli

Applications

Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

650 ug/mL (prior to lyoph)

Format

Lyophilized// PBS (pH 7.4) with 0.01% SKL, 5% Trehalose

Reconstitution

Reconstitute in 10mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/ml. Do not vortex. Store at 2-8° C for one month. Aliquot and store at -80° C for 12 months. Avoid repeated freeze/thaw cycles.