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MGEA5 Recombinant Protein

Product Specifications

CAS Number

9000-83-3

Gene Name

O-GlcNAcase

Gene Aliases

Beta-hexosaminidase; beta-N-acetylglucosaminidase; beta-N-acetylhexosaminidase; bifunctional protein NCOAT; hyaluronidase in meningioma; MEA5; meningioma expressed antigen 5 (hyaluronidase) ; meningioma-expressed antigen 5; MGEA5; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; NCOAT; nuclear cytoplasmic O-GlcNAcase and acetyltransferase; protein O-GlcNAcase.

Gene ID

10724

Accession Number

NP_001135906.1

Reactivity

Homo sapiens|Human

Target

Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210) . Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714) .

Conjugation

Unconjugated

Type

Protein

Source

E.coli

Applications

Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

200 ug/mL (prior to lyoph)

Format

Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Reconstitution

2°C to 8°C|-80°C

Molecular Weight

20kDa

Protein Length

Recombinant

NCBI Gene Symbol

OGA

Protein Name

Protein O-GlcNAcase

Gene Name URL

OGA

Nucleotide Accession Number

NM_001142434.1

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