What is the catalog number for MGEA5 Recombinant Protein?

The catalog number for MGEA5 Recombinant Protein is OPCD05564-200UG.

What sizes are available for MGEA5 Recombinant Protein?

MGEA5 Recombinant Protein is available in the following size(s): 200 µg.

Is MGEA5 Recombinant Protein in stock?

MGEA5 Recombinant Protein is currently made to order / not held in immediate stock. Contact us for lead time.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

MGEA5 Recombinant Protein

Product Specifications

CAS Number

9000-83-3

Gene Name

O-GlcNAcase

Gene Aliases

Beta-hexosaminidase; beta-N-acetylhexosaminidase; bifunctional protein NCOAT; meningioma expressed antigen 5 (hyaluronidase) ; meningioma-expressed antigen 5; Mgea5; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; Ncoat; nuclear cytoplasmic O-GlcNAcase and acetyltransferase; protein O-GlcNAcase; protein O-GlcNAcase-like.

Gene ID

154968

Accession Number

NP_571979.1

Reactivity

Rat|Rattus norvegicus

Target

Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:8034696) . Does not bind acetyl-CoA and does not have histone acetyltransferase activity.

Conjugation

Unconjugated

Type

Protein

Source

E.coli

Applications

Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

200 ug/mL (prior to lyoph)

Format

Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Reconstitution

2°C to 8°C|-80°C

Molecular Weight

21kDa

Protein Length

Recombinant

NCBI Gene Symbol

Oga

Host or Source

E.coli

Protein Name

Protein O-GlcNAcase

Gene Name URL

Oga

Nucleotide Accession Number

NM_131904.1

Frequently Asked Questions

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