MGEA5 Recombinant Protein
Product Specifications
CAS Number
9000-83-3
Gene Name
O-GlcNAcase
Gene Aliases
Beta-hexosaminidase; beta-N-acetylhexosaminidase; bifunctional protein NCOAT; Hy5; meningioma expressed antigen 5 (hyaluronidase) ; meningioma-expressed antigen 5; Mgea; Mgea5; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; Ncoat; nuclear cytoplasmic O-GlcNAcase and acetyltransferase; protein O-GlcNAcase.
Gene ID
76055
Accession Number
NP_076288.1
Reactivity
Mouse|Mus musculus
Target
Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:16517082) . Does not bind acetyl-CoA and does not have histone acetyltransferase activity.
Conjugation
Unconjugated
Type
Protein
Source
E.coli
Applications
Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
200 ug/mL (prior to lyoph)
Format
Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Reconstitution
2°C to 8°C|-80°C
Molecular Weight
17kDa
Protein Length
Recombinant
NCBI Gene Symbol
Oga
Host or Source
E.coli
Protein Name
Protein O-GlcNAcase
Gene Name URL
Oga
Nucleotide Accession Number
NM_023799.3
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