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GATA4 Recombinant Protein

Product Specifications

CAS Number

9000-83-3

Gene Name

GATA binding protein 4

Gene Aliases

DNA-binding protein GATA-GT2; GATA-binding factor 4; transcription factor GATA-4.

Gene ID

54254

Accession Number

NP_653331.1

Reactivity

Rat|Rattus norvegicus

Target

Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (By similarity) . In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression (By similarity) . Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity) . Promotes cardiac myocyte enlargement (By similarity) . Required during testicular development (By similarity) . Involved in bone morphogenetic protein (BMP) -mediated induction of cardiac-specific gene expression (PubMed:15329343) . Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (PubMed:15329343) . May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (By similarity) .

Conjugation

Unconjugated

Type

Protein

Source

E.coli

Applications

Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

150 ug/mL (prior to lyoph)

Format

Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Reconstitution

2°C to 8°C|-80°C

Molecular Weight

33kDa

Protein Length

Recombinant

NCBI Gene Symbol

Gata4

Host or Source

E.coli

Protein Name

Transcription factor GATA-4

Gene Name URL

Gata4

Nucleotide Accession Number

NM_144730.1

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