FBXW7 Recombinant Protein

Product Specifications

CAS Number

9000-83-3

Gene Name

F-box and WD-40 domain protein 7

Gene Aliases

1110001A17Rik; A; AGO; Cdc4; F-box and WD-40 domain protein 7, archipelago homolog; F-box and WD-40 domain-containing protein 7; f-box only protein 30; F-box protein FBW7; F-box protein Fbxw6; F-box/WD repeat-containing protein 7; F-box-WD40 repeat protein 6; Fbw7; Fbwd6; Fbx30; Fbxo3; Fbxo30; Fbxw6; SEL-; SEL-10.

Gene ID

50754

Accession Number

NP_536353.2

Reactivity

Mouse|Mus musculus

Target

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:21953459) . Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (By similarity) . Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NOTCH2, MCL1 and probably PSEN1 (By similarity) . Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (By similarity) . SCF (FBXW7) complex mediates the ubiquitination and subsequent degradation of NFE2L1 (PubMed:21953459) . Involved in bone homeostasis and negative regulation of osteoclast differentiation (PubMed:29149593) .

Conjugation

Unconjugated

Type

Protein

Source

E.coli

Applications

Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol