FBXW7 Recombinant Protein
Product Specifications
CAS Number
9000-83-3
Gene Name
F-box and WD repeat domain containing 7
Gene Aliases
AGO; archipelago; Archipelago homolog; CDC4; F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase; F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila) ; F-box and WD-40 domain-containing protein 7; F-box protein FBW7; F-box protein FBX30; F-box protein SEL-10; F-box/WD repeat-containing protein 7; FBW6; FBW7; FBX30; FBXO30; FBXW6; hAgo; hCdc4; homolog of C elegans sel-10; SEL10; SEL-10.
Gene ID
55294
Accession Number
NP_001013433.1
Reactivity
Homo sapiens|Human
Target
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (PubMed:17434132) . Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NOTCH2, MCL1, and probably PSEN1 (PubMed:11565034, PubMed:12354302, PubMed:11585921, PubMed:15103331, PubMed:14739463, PubMed:17558397, PubMed:17873522, PubMed:22608923, PubMed:29149593, PubMed:25775507, PubMed:28007894) . Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (PubMed:14739463) . SCF (FBXW7) complex mediates the ubiquitination and subsequent degradation of NFE2L1 (By similarity) . Involved in bone homeostasis and negative regulation of osteoclast differentiation (PubMed:29149593) .
Conjugation
Unconjugated
Type
Protein
Source
E.coli
Applications
Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
200 ug/mL (prior to lyoph)
Format
Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Reconstitution
2°C to 8°C|-80°C
Molecular Weight
31kDa
Protein Length
Recombinant
NCBI Gene Symbol
FBXW7
Host or Source
E.coli
Protein Name
F-box/WD repeat-containing protein 7
Gene Name URL
FBXW7
Nucleotide Accession Number
NM_001013415.1
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