EP300 Recombinant Protein

A430090G16; A730011L11; E1A-associated protein p300; histone acetyltransferase p300; histone butyryltransferase p300; histone crotonyltransferase p300; KAT3; KAT3B; p30; p300; p300 HAT; protein 2-hydroxyisobutyryltransferase p300; protein lactyltransferas p300; protein propionyltransferase p300.

Functions as histone acetyltransferase and regulates transcription via chromatin remodeling (By similarity) . Acetylates all four core histones in nucleosomes (By similarity) . Histone acetylation gives an epigenetic tag for transcriptional activation (By similarity) . Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein (PubMed:18486321, PubMed:24216764) . Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability (By similarity) . Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac) (By similarity) . Also functions as acetyltransferase for nonhistone targets (By similarity) . Acetylates 'Lys-131' of ALX1 and acts as its coactivator (By similarity) . Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function (By similarity) . Acetylates HDAC1 leading to its inactivation and modulation of transcription (By similarity) . Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2 (By similarity) . Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium (By similarity) . Promotes cardiac myocyte enlargement (By similarity) . Can also mediate transcriptional repression (By similarity) . Acetylates FOXO1 and enhances its transcriptional activity (By similarity) . Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity (By similarity) . Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter (By similarity) . Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity (PubMed:14645221, PubMed:9512516) . Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity (PubMed:20955178) . Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (By similarity) . Acetylates MEF2D (By similarity) . Acetylates and stabilizes ZBTB7B protein by antagonizing ubiquitin conjugation and degragation, this mechanism may be involved in CD4/CD8 lineage differentiation (PubMed:20810990) . In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as (2E) -butenoyl-CoA (crotonyl-CoA), butanoyl-CoA (butyryl-CoA) or propanoyl-CoA (propionyl-CoA), and is able to mediate protein crotonylation, butyrylation or propionylation, respectively (PubMed:27105113) . Acts as a histone crotonyltransferase; crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Histone crotonyltransferase activity is dependent on the concentration of (2E) -butenoyl-CoA (crotonyl-CoA) substrate and such activity is weak when (E) -but-2-enoyl-CoA (crotonyl-CoA) concentration is low (By similarity) . Also acts as a histone butyryltransferase; butyrylation marks active promoters (PubMed:27105113) .

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol