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EIF4EBP1 Recombinant Protein

Product Specifications

CAS Number

9000-83-3

Gene Name

Eukaryotic translation initiation factor 4E binding protein 1

Gene Aliases

4E-BP1; eIF4E-binding protein 1; eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; phosphorylated heat- and acid-stable protein regulated by insulin 1.

Gene ID

116636

Accession Number

NP_446309.1

Reactivity

Rat|Rattus norvegicus

Target

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation (By similarity) . Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways (PubMed:7939721) .

Conjugation

Unconjugated

Type

Protein

Source

E.coli

Applications

Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

200 ug/mL (prior to lyoph)

Format

Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Reconstitution

2°C to 8°C|-80°C

Molecular Weight

20kDa

Protein Length

Recombinant

NCBI Gene Symbol

Eif4ebp1

Host or Source

E.coli

Protein Name

Eukaryotic translation initiation factor 4E-binding protein 1

Gene Name URL

Eif4ebp1

Nucleotide Accession Number

NM_053857.2

Frequently Asked Questions

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