DNASE1 Recombinant Protein

Product Specifications

CAS Number

9000-83-3

Gene Name

Deoxyribonuclease 1

Gene Aliases

Deoxyribonuclease I; deoxyribonuclease-1; DNase I, lysosomal; DNL1; Dornase alfa; DRNI; human urine deoxyribonuclease I.

Gene ID

1773

Accession Number

NP_005214.2

Reactivity

Homo sapiens|Human

Target

Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed:2251263, PubMed:11241278, PubMed:2277032) . Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity) . Among other functions, seems to be involved in cell death by apoptosis (PubMed:11241278) . Binds specifically to G-actin and blocks actin polymerization (By similarity) . Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity) . NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity) . Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity) .

Type

Protein

Source

E.coli

Applications

Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol