ADAM10 Recombinant Protein

Product Specifications

CAS Number

9000-83-3

Gene Name

ADAM metallopeptidase domain 10

Gene Aliases

A disintegrin and metalloprotease domain 10; AD10; AD18; CD156c; CDw156; disintegrin and metalloproteinase domain-containing protein 10; HsT18717; kuz; kuzbanian protein homolog; MADM; mammalian disintegrin-metalloprotease; RAK.

Gene ID

102

Accession Number

NP_001101.1

Reactivity

Homo sapiens|Human

Target

Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (PubMed:20592283) . Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP) (PubMed:26686862, PubMed:11786905, PubMed:29224781) . Contributes to the normal cleavage of the cellular prion protein (PubMed:11477090) . Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity (PubMed:12475894) . Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis (By similarity) . Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form (PubMed:17557115) . Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B (PubMed:19114711, PubMed:21288900) . May regulate the EFNA5-EPHA3 signaling (PubMed:16239146) .

Conjugation

Unconjugated

Type

Protein

Source

E.coli

Applications

Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol