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MED1 Recombinant Protein

Product Specifications

CAS Number

9000-83-3

Gene Name

Mediator complex subunit 1

Gene Aliases

Activator-recruited cofactor 205 kDa component; ARC205; CRSP1; CRSP200; DRIP205; DRIP230; Mediator complex subunit 1; mediator of RNA polymerase II transcription subunit 1; p53 regulatory protein RB18A; PBP; peroxisome proliferator-activated receptor-binding protein; PPAR-binding protein; PPARBP; PPARG binding protein; PPARGBP; RB18A; thyroid hormone receptor-associated protein complex 220 kDa component; thyroid hormone receptor-associated protein complex component TRAP220; thyroid receptor interacting protein 2; Thyroid receptor-interacting protein 2; TRAP220; TR-interacting protein 2; TRIP2; TRIP-2; vitamin D receptor-interacting protein 230 kD; vitamin D receptor-interacting protein complex component DRIP205.

Gene ID

5469

Accession Number

NP_004765.2

Reactivity

Homo sapiens|Human

Target

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed:10406464, PubMed:11867769, PubMed:12037571, PubMed:12218053, PubMed:12556447, PubMed:14636573, PubMed:15340084, PubMed:15471764, PubMed:15989967, PubMed:16574658, PubMed:9653119) . Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781) .

Conjugation

Unconjugated

Type

Protein

Source

E.coli

Applications

Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

200 ug/mL (prior to lyoph)

Format

Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

Reconstitution

2°C to 8°C|-80°C

Molecular Weight

28kDa

Protein Length

Recombinant

NCBI Gene Symbol

MED1

Host or Source

E.coli

Protein Name

Mediator of RNA polymerase II transcription subunit 1

Gene Name URL

MED1

Nucleotide Accession Number

NM_004774.3

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