AER Recombinant Protein (Mouse-ear cress)

Alkenal reductase; AT5G16970; AT-AER; F2K13.120; F2K13_120; NADP-dependent alkenal double bond reductase P1; NADPH:2-alkenal/one alpha, beta-hydrogenase; NADPH-azodicarbonyl/quinone reductase; P1-zeta-crystallin protein.

Involved in the detoxification of reactive carbonyls (PubMed:10848984, PubMed:12514241, PubMed:16299173) . Acts on lipid peroxide-derived reactive aldehydes (PubMed:12514241) . Specific to a double bond activated by an adjacent carbonyl group (PubMed:12514241) . Can use both quinones and diamide as substrates, but not menadione, ferricyanide or phylloquinone (PubMed:10848984) . Can use 4-hydroxy- (2E) -nonenal (HNE), 4-hydroxy- (2E) -hexenal (HHE), (2E) -nonenal, (2E) -hexenal, (2E) -pentenal, propenal (acrolein), 3-buten-2-one and 3-penten-2-one, but not (R) - (-) -carvone, n-nonanal, n-hexanal, (3Z) -hexanal, cyclohex-2-en-1-one or 12-oxo phytodienoic acid (OPDA) as electron acceptors (PubMed:12514241) . Catalyzes the reduction of the alpha, beta-unsaturated bond of 2-alkenals, of lipid peroxide-derived oxenes 9-oxo-10 (E),12 (Z) -octadecadienoic acid (9-KODE) and 13-oxo-9 (Z),11 (E) -octadecadienoic acid (13-KODE), as well as 4-oxo- (2E) -nonenal and 4-hydroxynonenal (PubMed:16299173) . Can use 12-oxo-10 (E) dodecanoate (traumatin), trans-1,3 diphenyl-2-propenone, trans-1,4-diphenyl-2-butene-1,4-dione, 9-oxo-12,13-epoxy- (10E) -octadecenoic acid (trans-EKODE-1b) and 9,13-dihydroxy-10-oxo-11-octadecenoic acid as substrates (PubMed:26678323) . Catalyzes the reduction of the 7-8 double bond of phenylpropanal substrates, such as p-coumaryl aldehyde and coniferyl aldehyde (in vitro) (PubMed:17028190) . Has activity towards toxic substrates, such as 4-hydroxy- (2E) -nonenal (in vitro) (PubMed:17028190) . May play a distinct role in plant antioxidant defense and is possibly involved in NAD (P) /NAD (P) H homeostasis (PubMed:17028190) .

MTATNKQVILKDYVSGFPTESDFDFTTTTVELRVPEGTNSVLVKNLYLSCDPYMRIRMGKPDPSTAALAQAYTPGQPIQGYGVSRIIESGHPDYKKGDLLWGIVAWEEYSVITPMTHAHFKIQHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQLAKMMGCYVVGSAGSKEKVDLLKTKFGFDDAFNYKEESDLTAALKRCFPNGIDIYFENVGGKMLDAVLVNMNMHGRIAVCGMISQYNLENQEGVHNLSNIIYKRIRIQGFVVSDFYDKYSKFLEFVLPHIREGKITYVEDVADGLEKAPEALVGLFHGKNVGKQVVVVARE

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.