LasB Recombinant Protein
Cleaves host elastin, collagen, IgG, and several complement components as well as endogenous pro-aminopeptidase (PubMed:11533066) . Autocatalyses processing of its pro-peptide (PubMed:9642203, PubMed:1744034) . Processes the pro-peptide of pro-chitin-binding protein (cbpD) (PubMed:9642203) . Involved in the pathogenesis of P.aeruginosa infections.
Product Specifications
CAS Number
9000-83-3
Gene Name
Elastase LasB
Gene Aliases
Elastase LasB; Neutral metalloproteinase; PA3724; PAE; Pseudolysin.
Gene ID
880368
Accession Number
NP_252413.1
Reactivity
Pseudomonas aeruginosa
Target
Cleaves host elastin, collagen, IgG, and several complement components as well as endogenous pro-aminopeptidase (PubMed:11533066) . Autocatalyses processing of its pro-peptide (PubMed:9642203, PubMed:1744034) . Processes the pro-peptide of pro-chitin-binding protein (cbpD) (PubMed:9642203) . Involved in the pathogenesis of P.aeruginosa infections.
Type
Protein
Source
E.coli
Sequence
AEAGGPGGNQKIGKYTYGSDYGPLIVNDRCEMDDGNVITVDMNSSTDDSKTTPFRFACPTNTYKQVNGAYSPLNDAHFFGGVVFKLYRDWFGTSPLTHKLYMKVHYGRSVENAYWDGTAMLFGDGATMFYPLVSLDVAAHEVSHGFTEQNSGLIYRGQSGGMNEAFSDMAGEAAEFYMRGKNDFLIGYDIKKGSGALRYMDQPSRDGRSIDNASQYYNGIDVHHSSGVYNRAFYLLANSPGWDTRKAFEVFVDANRYYWTATSNYNSGACGVIRSAQNRNYSAADVTRAFSTVGVTCPSAL
Purification
Affinity purified using IMAC.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Format
Liquid or Lyophilized powder
Reconstitution
-20°C or -80°C
Molecular Weight
49.1 kDa
Protein Length
Recombinant
NCBI Gene Symbol
LasB
Host or Source
Pseudomonas aeruginosa
Protein Name
Elastase
Gene Name URL
LasB
Nucleotide Accession Number
NC_002516.2
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