Ripk3 Recombinant Protein

Product Specifications

CAS Number

9000-83-3

Gene Name

Receptor-interacting serine-threonine kinase 3

Gene Aliases

2610528K09Rik; AW107945; mRIP3; R; receptor interacting protein 3; Receptor-interacting protein 3; receptor-interacting serine/threonine-protein kinase 3; Rip3; RIP-3; RIP-like protein kinase 3.

Gene ID

56532

Accession Number

NP_064339.2

Reactivity

Mouse|Mus musculus

Target

Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.

Type

Protein

Source

Yeast

Purification

Affinity purified using IMAC

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

Varies by lot. See vial for concentration.

Format

Liquid// 20mM Tris-HCl, 0.5M NaCl (pH 8.0), 50% glycerol

Reconstitution

Store at -20° C; for extended storage, conserve at -20° C or -80° C. Store at 4° C for up to one week. Repeated freeze/thaw cycles are not recommended.