Trypsin Activity Assay Kit (Fluorometric)

Trypsin (EC 3.4.21.4) is a serine protease that is expressed in bacteria, fungi and mammals. Trypsin cleaves peptide chains at the carboxyl side of the amino acids namely arginine and lysine. In mammals, trypsin is secreted from the pancreas as an inactive precursor (trypsinogen) and is processed to its active form in the small intestine, where it plays an essential role in protein hydrolysis and absorption. Activation of trypsin in pancreas can lead to a series of events thereby resulting in pancreatitis. Trypsin is therefore a useful biomarker for pancreatitis. Additionally, monitoring trypsin activity plays a central role in biochemical, pharmaceutical and has clinical applications. BioVision’s Trypsin Activity Assay Kit provides a simple, rapid way to detect trypsin activity in a wide variety of biological samples. In this kit, trypsin cleaves a synthetic substrate thereby releasing a fluorophore (AMC), which can be easily quantified at Ex/Em = 380/460 nm. By pretreating samples at 60ºC for 20 min, the kit can differentiate the trypsin activity from other structurally similar enzymes such as chymotrypsin and tryptase. The assay is specific, sensitive and can detect as low as 2 µU of trypsin activity in samples.