1.0 M HEPES pH 7.2

HEPES is a useful buffer for pH's ranging from 6.8-8.2. This makes HEPES ideal for cell culturing because of its usefulness in maintaining pH changes in presence of carbon dioxide production from cellular respiration. 1.0 M HEPES pH 7.2 is ideal for investigators in Molecular Biology, Cell Biology, and Immunology researchers._x005F

1.0 HEPES pH 7.2 was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. 1.0 M HEPES pH 7.2 was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Store container at room temperature (18-26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18-26° C) to restore solubility of some salts. 1.0 M HEPES pH 7.2 is a concentrated stock solution and should be diluted appropriately with molecular biology grade water or equivalent to its final working concentration.