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10X PBS pH 7.2 with Azide

Product Specifications

Target

Phosphate Buffered Saline (PBS) is a regularly used buffer in biological research. The phosphate ions help maintain a constant pH around neutral. The osmolarity of solution typically matches that of the human body (isotonic solution) . PBS can act as a diluent for many experimental preparations and is non-toxic to cells. This preparation contains 0.1% sodium azide as a preservative and is 10X concentrated to be diluted 10-fold to the working concentration._x005F

Conjugation

Unconjugated

Type

Others

Applications

Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

Purification

Aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

10X

Format

Liquid (sterile filtered)

Reconstitution

Store container at room temperature (18 to 26° C) . If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18 to 26° C) to restore solubility of some salts. Expiration date is one (1) year from date of opening.

Specificity

Tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

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