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STREPTAVIDIN

Product Specifications

Gene Aliases

SA, S avidin, streptococcus avidin

Reactivity

Streptavidin, Streptomyces avidinii

Target

Streptavidin

Conjugation

Unconjugated

Type

Others

Applications

Chromatin Immunoprecipitation, Enzyme-linked immunosorbent assay, Gel shift assay, Immunofluorescence, Microarray, Sodium sodecyl sulfate - polyacrylamide gel electrophoresis, Western blot

Purification

Streptavidin is chromatographically pure.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1.0 mg/mL by UV absorbance at 280 nm (prior to lyophilization)

Bioactivity

15.5 U/mg by biotin titration method

Format

Lyophilized

Reconstitution

Restore with deionized water (or equivalent) and reconstitution volume is 5.0 mL. Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Streptavidin is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration is 1 year from date of opening.

Specificity

Shows predominantly a single 53,600 dalton band by SDS-PAGE.

Protein Name

P22629

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