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AVIDIN GLUCOSE OXIDASE Conjugated

Avidin is a biotin-binding protein found in the oviducts of egg-laying animals (birds, reptiles, and frogs) that gets deposited into the whites of their eggs. Avidin is a tetramer and can bind up to four biotin molecules (Vitamin B7) with one of the greatest known non-covalent interactions. Avidity for biotin is destroyed with heat. Glucose oxidase is an enzyme that drives the oxidation of glucose into hydrogen peroxide and D-glucono-delta-lactone. This activity is utilized in biotechnology through colormetric detection assays that are sensitive to the amount of hydrogen peroxide produced.

Product Specifications

Reactivity

Avidin, Gallus gallus

Target

Avidin

Conjugation

Glucose Oxidase

Type

Others

Applications

Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

Purification

Avidin Glucose Oxidase was prepared from chromatographically pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

2 mg/ml

Format

Lyophilized

Reconstitution

2°C to 8°C, -20°C or -80°C

Molecular Weight

160 kDa

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin and anti-Glucose Oxidase.

Protein Name

P02701

Frequently Asked Questions

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