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M CSF Protein

Product Specifications

Gene Aliases

CSF-1, Lanimostim, MCSF, MGC31930, M-CSF.

Reactivity

Rat

Target

M-CSF is disulfide-linked homodimer containing 2 x 222 a.a. chains.

Type

Protein

Source

HEK293 Cells

Purification

Greater than 95.0% as determined by SDS-PAGE.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Bioactivity

The ED50 of 3-4ng/ml is measured by its ability to stimulate the proliferation of mouse M-NFS-60 cells.

Format

Lyophilized// after extensive dialysis against PBS

Reconstitution

It is recommended to reconstitute the MCSF in sterile PBS not less than 100 ug/mL, which can be then be further diluted to other aqueous solutions. Lyophilized MCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18° C. Upon reconstitution MCSF should be stored at 4° C between 2-7 days and for future use below -18° C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Notes

Formerly GWB-BSP060

Protein Length

Recombinant

NCBI Gene Symbol

M CSF

Host or Source

HEK293

Gene Name URL

M CSF

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