M CSF Protein
Product Specifications
Gene Aliases
CSF-1, Lanimostim, MCSF, MGC31930, M-CSF.
Reactivity
Rat
Target
M-CSF is disulfide-linked homodimer containing 2 x 222 a.a. chains.
Type
Protein
Source
HEK293 Cells
Purification
Greater than 95.0% as determined by SDS-PAGE.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Bioactivity
The ED50 of 3-4ng/ml is measured by its ability to stimulate the proliferation of mouse M-NFS-60 cells.
Format
Lyophilized// after extensive dialysis against PBS
Reconstitution
It is recommended to reconstitute the MCSF in sterile PBS not less than 100 ug/mL, which can be then be further diluted to other aqueous solutions. Lyophilized MCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18° C. Upon reconstitution MCSF should be stored at 4° C between 2-7 days and for future use below -18° C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.
Notes
Formerly GWB-BSP060
Protein Length
Recombinant
NCBI Gene Symbol
M CSF
Host or Source
HEK293
Gene Name URL
M CSF
Frequently Asked Questions
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