Neopterin ELISA kit (Human)

2-amino-6- (1,2,3-trihydroxypropyl) -1H-pteridin-4-one, D- (+) -Neopterin, D-Neopterin, Neopterin, D-erythro-Neopterin, 2009-64-5, 6-D-erythro-Neopterin

Neopterin is a catabolic product of guanosine triphosphate (GTP), a purine nucleotide._x005F Neopterin belongs to the chemical group known as pteridines. It is synthesised by human macrophages upon stimulation with the cytokine interferon-gamma and is indicative of a pro-inflammatory immune status. Neopterin serves as a marker of cellular immune system activation.

Aviva Systems Biology Neopterin ELISA Kit (Human) (OKEH00637) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Neopterin antibody and blocked. Sample or standards are added to the wells along with a fixed quantity of biotinylated Neopterin and incubated. The Neopterin found in the sample or standards competes with the biotinylated Neopterin for limited binding sites on the immobilized anti-Neopterin antibody. Excess unbound biotinylated Neopterin and sample or standard Neopterin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Neopterin captured in the well and inversely proportional to the amount of Neopterin which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Amount _x005F Neopterin Microplate 96 Wells (12 x 8 Well Strips) _x005F Neopterin Lyophilized Standard 2 x 20 ng _x005F 100X Neopterin Biotin Complex 120 uL _x005F Avidin/HRP Conjugate 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Complex Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL Component Amount Component Amount Neopterin Microplate 96 Wells (12 x 8 Well Strips) Neopterin Microplate 96 Wells (12 x 8 Well Strips) Neopterin Lyophilized Standard 2 x 20 ng Neopterin Lyophilized Standard 2 x 20 ng 100X Neopterin Biotin Complex 120 uL 100X Neopterin Biotin Complex 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Complex Diluent 1 x 12 mL Complex Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL