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IGFI ELISA Kit (Turkey)

Product Specifications

Reproducibility

Mean Inter-Assay Precision

CAS Number

7732-18-5

Gene Aliases

IGF1

Gene ID

678666

Accession Number

NP_001290078

Reactivity

Turkey

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Igfi ELISA Kit (Turkey) (OKWB00442) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Igfi has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Igfi is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Igfi captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

Detection Range

0.156 - 10 ng/mL

Recovery

Sample Type Range Mean Serum (n=5) 88-105 96 EDTA Plasma (n=5) 87-101 91 Heparin Plasma (n=5) 89-104 99 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 88-105 96 Serum (n=5) 88-105 96 EDTA Plasma (n=5) 87-101 91 EDTA Plasma (n=5) 87-101 91 Heparin Plasma (n=5) 89-104 99 Heparin Plasma (n=5) 89-104 99

Sensitivity

0.094 ng/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

IGFI

Protein Name

Insulin-like growth factor-I

Gene Name URL

IGFI

Nucleotide Accession Number

NM_001303149

Frequently Asked Questions

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