OREXIN A AND B ELISA Kit (Sheep)

Aviva Systems Biology Orexin a and b ELISA Kit (Sheep) (OKWB00437) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Orexin a and b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Orexin a and b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Orexin a and b captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type Range Mean _x005F Serum (n=5) 86-102 92 _x005F EDTA Plasma (n=5) 87-104 95 _x005F Heparin Plasma (n=5) 85-105 97 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-102 92 Serum (n=5) 86-102 92 EDTA Plasma (n=5) 87-104 95 EDTA Plasma (n=5) 87-104 95 Heparin Plasma (n=5) 85-105 97 Heparin Plasma (n=5) 85-105 97

Component Amount _x005F Orexin a and b Microplate 96 Wells (12 x 8 Well strips) _x005F Orexin a and b Lyophilized Standard 2 x 1 ug _x005F 100X Orexin a and b Biotinylated Detector Anitbody 1 x 120 uL _x005F 100X Avidin-HRP Conjugate 1 x 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 25X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 10 mL _x005F Stop Solution 1 x 10 mL Component Amount Component Amount Orexin a and b Microplate 96 Wells (12 x 8 Well strips) Orexin a and b Microplate 96 Wells (12 x 8 Well strips) Orexin a and b Lyophilized Standard 2 x 1 ug Orexin a and b Lyophilized Standard 2 x 1 ug 100X Orexin a and b Biotinylated Detector Anitbody 1 x 120 uL 100X Orexin a and b Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL