UOX ELISA Kit (Rabbit)
Product Specifications
Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Aliases
UOX
Gene ID
100009266
Accession Number
NP_001121545
Reactivity
Rabbit
Target
Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S) -allantoin.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Uox ELISA Kit (Rabbit) (OKWB00424) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Uox has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Uox is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Uox captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
0.156 - 10 ng/mL
Recovery
Sample Type Range Mean Serum (n=5) 87-102 92 EDTA Plasma (n=5) 86-105 96 Heparin Plasma (n=5) 85-104 92 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 87-102 92 Serum (n=5) 87-102 92 EDTA Plasma (n=5) 86-105 96 EDTA Plasma (n=5) 86-105 96 Heparin Plasma (n=5) 85-104 92 Heparin Plasma (n=5) 85-104 92
Sensitivity
0.094 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
UOX
Protein Name
Uricase
Gene Name URL
UOX
Nucleotide Accession Number
NM_001128073
Frequently Asked Questions
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