IL5 ELISA Kit (Rabbit)

Product Specifications

Reproducibility

Mean Inter-Assay Precision

CAS Number

7732-18-5

Gene Name

Interleukin 5

Gene Aliases

Eosinophil differentiation factor; interleukin-5.

Gene ID

100358075

Accession Number

XP_002710247

Reactivity

Oryctolagus cuniculus|Rabbit

Target

Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Detection Method

Colorimetric, OD 450 nm

Assay Principle

Aviva Systems Biology IL5 ELISA Kit (Rabbit) (OKWB00411) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL5 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

Detection Range

15.625-1000pg/ml

Recovery

Sample Type Range Mean Serum (n=5) 85-105 96 EDTA Plasma (n=5) 87-105 94 Heparin Plasma (n=5) 85-103 91 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 85-105 96 Serum (n=5) 85-105 96 EDTA Plasma (n=5) 87-105 94 EDTA Plasma (n=5) 87-105 94 Heparin Plasma (n=5) 85-103 91 Heparin Plasma (n=5) 85-103 91

Sensitivity

9.4 pg/mL

Reconstitution

Store as indicated in product manual.

Components

Component Amount IL5 Microplate 96 Wells (12 x 8 Well strips) IL5 Lyophilized Standard 2 x 1 ng 100X IL5 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount IL5 Microplate 96 Wells (12 x 8 Well strips) IL5 Microplate 96 Wells (12 x 8 Well strips) IL5 Lyophilized Standard 2 x 1 ng IL5 Lyophilized Standard 2 x 1 ng 100X IL5 Biotinylated Detector Anitbody 1 x 120 uL 100X IL5 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL