What is the catalog number for MTL5 ELISA Kit (Rat)?

The catalog number for MTL5 ELISA Kit (Rat) is OKWB00383-96W.

What sizes are available for MTL5 ELISA Kit (Rat)?

MTL5 ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is MTL5 ELISA Kit (Rat) in stock?

MTL5 ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

Is this product classed as hazardous or restricted for shipping?

Some products are subject to special import/export regulations, dangerous goods handling, or licensing requirements. Where this applies, it’s noted on the product page, and any required extra documents or fees will be confirmed with you before dispatch, never added without your consent.

MTL5 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay Precision

CAS Number

7732-18-5

Gene Name

Testis expressed metallothionein like protein

Gene Aliases

Metallothionein-like 5, testis-specific; metallothionein-like 5, testis-specific (tesmin) ; Mtl5; tesmin.

Gene ID

309142

Accession Number

NP_001012069

Reactivity

Rat|Rattus norvegicus

Target

May have a role in spermatogenesis.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Detection Method

Colorimetric, OD 450 nm

Assay Principle

Aviva Systems Biology Mtl5 ELISA Kit (Rat) (OKWB00383) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mtl5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mtl5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mtl5 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

Detection Range

31.25-2000pg/ml

Recovery

Sample Type Range Mean Serum (n=5) 88-102 96 EDTA Plasma (n=5) 87-100 92 Heparin Plasma (n=5) 86-101 93 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 88-102 96 Serum (n=5) 88-102 96 EDTA Plasma (n=5) 87-100 92 EDTA Plasma (n=5) 87-100 92 Heparin Plasma (n=5) 86-101 93 Heparin Plasma (n=5) 86-101 93

Sensitivity

18.75 pg/mL

Reconstitution

Store as indicated in product manual.

Components

Component Amount Mtl5 Microplate 96 Wells (12 x 8 Well strips) Mtl5 Lyophilized Standard 2 x 2 ng 100X Mtl5 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Mtl5 Microplate 96 Wells (12 x 8 Well strips) Mtl5 Microplate 96 Wells (12 x 8 Well strips) Mtl5 Lyophilized Standard 2 x 2 ng Mtl5 Lyophilized Standard 2 x 2 ng 100X Mtl5 Biotinylated Detector Anitbody 1 x 120 uL 100X Mtl5 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL