What is the catalog number for XPNPEP1 ELISA Kit (Rat)?

The catalog number for XPNPEP1 ELISA Kit (Rat) is OKWB00343-96W.

What sizes are available for XPNPEP1 ELISA Kit (Rat)?

XPNPEP1 ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is XPNPEP1 ELISA Kit (Rat) in stock?

XPNPEP1 ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

Do you offer bulk or custom quantities of this product?

Yes. Ordering two or more units, or combining this with other products, usually qualifies for a discount depending on quantity. Contact our sales team with your required volume for a custom quote.

Do you provide a Certificate of Analysis (CoA) for this product?

A Certificate of Analysis is available for most products upon request. Contact our support team with the lot number to obtain the relevant CoA.

Is this product suitable for live cell imaging?

Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.

XPNPEP1 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay Precision

CAS Number

7732-18-5

Gene Aliases

Aminoacylproline aminopeptidase, App, Cytosolic aminopeptidase P, sAmp, Soluble aminopeptidase P, Xaa-Pro aminopeptidase 1, X-Pro aminopeptidase 1, X-prolyl aminopeptidase 1, soluble

Gene ID

170751

Accession Number

NP_571988

Reactivity

Rat

Target

Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Xpnpep1 ELISA Kit (Rat) (OKWB00343) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Xpnpep1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Xpnpep1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Xpnpep1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

Detection Range

1.563 - 100 ng/mL

Recovery

Sample Type Range Mean Serum (n=5) 88-100 93 EDTA Plasma (n=5) 93-105 98 Heparin Plasma (n=5) 85-102 96 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 88-100 93 Serum (n=5) 88-100 93 EDTA Plasma (n=5) 93-105 98 EDTA Plasma (n=5) 93-105 98 Heparin Plasma (n=5) 85-102 96 Heparin Plasma (n=5) 85-102 96