GDNF ELISA Kit (Pig)

Product Specifications

Reproducibility

Mean Inter-Assay Precision

CAS Number

7732-18-5

Reactivity

Porcine|Sus scrofa

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Detection Method

Colorimetric, OD 450 nm

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

Detection Range

0.156-10ng/ml

Sensitivity

0.094 ng/mL

Reconstitution

Store as indicated in product manual.

Components

Component Amount _x005F Gdnf Microplate 96 Wells (12 x 8 Well strips) _x005F Gdnf Lyophilized Standard 2 x 10 ng _x005F 100X Gdnf Biotinylated Detector Anitbody 1 x 120 uL _x005F 100X Avidin-HRP Conjugate 1 x 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 25X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 10 mL _x005F Stop Solution 1 x 10 mL Component Amount Component Amount Gdnf Microplate 96 Wells (12 x 8 Well strips) Gdnf Microplate 96 Wells (12 x 8 Well strips) Gdnf Lyophilized Standard 2 x 10 ng Gdnf Lyophilized Standard 2 x 10 ng 100X Gdnf Biotinylated Detector Anitbody 1 x 120 uL 100X Gdnf Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL