FURIN ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Name
Furin (paired basic amino acid cleaving enzyme)
Gene Aliases
9130404I01Rik; dibasic-processing enzyme; Fu; Fur; furin; PA; PACE; paired basic amino acid residue cleaving enzyme; Paired basic amino acid residue-cleaving enzyme; Pcs; Pcsk3; prohormone convertase 3; proprotein convertase subtilisin/kexin type 3; SP; SPC1; subtilisin pro-protein processing enzyme.
Gene ID
18550
Accession Number
NP_001074923
Reactivity
Mouse|Mus musculus
Target
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology Furin ELISA Kit (Mouse) (OKWB00272) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Furin has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Furin is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Furin captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
46.875-3000pg/ml
Recovery
Sample Type Range Mean Serum (n=5) 85-100 93 EDTA Plasma (n=5) 85-104 95 Heparin Plasma (n=5) 85-105 98 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 85-100 93 Serum (n=5) 85-100 93 EDTA Plasma (n=5) 85-104 95 EDTA Plasma (n=5) 85-104 95 Heparin Plasma (n=5) 85-105 98 Heparin Plasma (n=5) 85-105 98
Sensitivity
28.1 pg/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount Furin Microplate 96 Wells (12 x 8 Well strips) Furin Lyophilized Standard 2 x 3 ng 100X Furin Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Furin Microplate 96 Wells (12 x 8 Well strips) Furin Microplate 96 Wells (12 x 8 Well strips) Furin Lyophilized Standard 2 x 3 ng Furin Lyophilized Standard 2 x 3 ng 100X Furin Biotinylated Detector Anitbody 1 x 120 uL 100X Furin Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
Furin
Protein Name
Furin
Gene Name URL
Furin
Nucleotide Accession Number
NM_001081454
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog