NLRP1A ELISA Kit (Mouse)

CARD7; caspase recruitment domain protein; caspase recruitment domain-containing protein 7; death effector filament-forming Ced-4-like apoptosis protein; DEFCAP; Gm14; Gm15; N; NAC; NACHT, leucine rich repeat and PYD containing 1; NACHT, LRR and PYD containing protein 1; NACHT, LRR and PYD domains-containing protein 1a; NACHT/LRR/pyrin domain-containing protein 1; Nalp1; Nalp1a; NLR family, pyrin domain containing 1; NLR family, pyrin domain containing 1A, CAST/EiJ strain specific; NLR family, pyrin domain containing 1A, PWK/PhJ strain specific; NLR family, pyrin domain containing 1A, WSB/EiJ strain specific; Nlrp1; nucleotide-binding domain and caspase recruitment domain; PP1044.

As a potential sensor component of the NLRP1 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of Nlrp1b, CASP1, and possibly PYCARD. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP1 inflammasome is also required for HMGB1 secretion. The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (By similarity) (PubMed:23219391) . When activated in the bone marrow, induces the pyroptosis of hematopoietic stem cells and progenitor cells of both myeloid and lymphoid lineages, hence allowing the removal of damaged cells, and the release of IL1B, which induces granulopoiesis (PubMed:23219391) . Binds ATP (By similarity) .

Aviva Systems Biology Nlrp1a ELISA Kit (Mouse) (OKWB00267) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nlrp1a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nlrp1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nlrp1a captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type Range Mean Serum (n=5) 87-103 95 EDTA Plasma (n=5) 92-101 96 Heparin Plasma (n=5) 85-96 92 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 87-103 95 Serum (n=5) 87-103 95 EDTA Plasma (n=5) 92-101 96 EDTA Plasma (n=5) 92-101 96 Heparin Plasma (n=5) 85-96 92 Heparin Plasma (n=5) 85-96 92

Component Amount Nlrp1a Microplate 96 Wells (12 x 8 Well strips) Nlrp1a Lyophilized Standard 2 x 500 pg 100X Nlrp1a Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Nlrp1a Microplate 96 Wells (12 x 8 Well strips) Nlrp1a Microplate 96 Wells (12 x 8 Well strips) Nlrp1a Lyophilized Standard 2 x 500 pg Nlrp1a Lyophilized Standard 2 x 500 pg 100X Nlrp1a Biotinylated Detector Anitbody 1 x 120 uL 100X Nlrp1a Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL