GPSM1 ELISA Kit (Mouse)

Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G-protein signaling. Keeps G (i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction.

Aviva Systems Biology Gpsm1 ELISA Kit (Mouse) (OKWB00264) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gpsm1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gpsm1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Gpsm1 captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type Range Mean Serum (n=5) 86-100 95 EDTA Plasma (n=5) 87-100 94 Heparin Plasma (n=5) 96-102 100 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-100 95 Serum (n=5) 86-100 95 EDTA Plasma (n=5) 87-100 94 EDTA Plasma (n=5) 87-100 94 Heparin Plasma (n=5) 96-102 100 Heparin Plasma (n=5) 96-102 100

Component Amount Gpsm1 Microplate 96 Wells (12 x 8 Well strips) Gpsm1 Lyophilized Standard 2 x 2 ng 100X Gpsm1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Gpsm1 Microplate 96 Wells (12 x 8 Well strips) Gpsm1 Microplate 96 Wells (12 x 8 Well strips) Gpsm1 Lyophilized Standard 2 x 2 ng Gpsm1 Lyophilized Standard 2 x 2 ng 100X Gpsm1 Biotinylated Detector Anitbody 1 x 120 uL 100X Gpsm1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL