NMNAT1 ELISA Kit (Mouse)

2610529L11Rik;5730441G13Rik; D4Cole; D4Cole1e; naMN adenylyltransferase 1; nicotinamide mononucleotide adenylyl transferase; nicotinamide mononucleotide adenylyltransferase 1; nicotinamide/nicotinate mononucleotide adenylyltransferase 1; nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1; nicotinate-nucleotide adenylyltransferase 1; nm; NMN adenylyltransferase 1; NMN/NaMN adenylyltransferase 1; nmnat.

Catalyzes the formation of NAD (+) from nicotinamide mononucleotide (NMN) and ATP (PubMed:15381699) . Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency (By similarity) . Can use triazofurin monophosphate (TrMP) as substrate (By similarity) . Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD (+) (By similarity) . For the pyrophosphorolytic activity, prefers NAD (+) and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively (By similarity) . Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5 (By similarity) . Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (By similarity) . Fails to cleave phosphorylated dinucleotides NADP (+), NADPH and NaADP (+) (By similarity) . Protects against axonal degeneration following mechanical or toxic insults (PubMed:15310905, PubMed:16914673) . Delays axonal degeneration after axotomy. Results in a >10-fold increase in intact neurites 72 hours after injury (PubMed:16914673) .

Aviva Systems Biology Nmnat1 ELISA Kit (Mouse) (OKWB00261) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nmnat1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nmnat1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nmnat1 captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type Range Mean Serum (n=5) 90-105 96 EDTA Plasma (n=5) 85-100 92 Heparin Plasma (n=5) 85-104 98 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 90-105 96 Serum (n=5) 90-105 96 EDTA Plasma (n=5) 85-100 92 EDTA Plasma (n=5) 85-100 92 Heparin Plasma (n=5) 85-104 98 Heparin Plasma (n=5) 85-104 98

Component Amount Nmnat1 Microplate 96 Wells (12 x 8 Well strips) Nmnat1 Lyophilized Standard 2 x 1 ng 100X Nmnat1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Nmnat1 Microplate 96 Wells (12 x 8 Well strips) Nmnat1 Microplate 96 Wells (12 x 8 Well strips) Nmnat1 Lyophilized Standard 2 x 1 ng Nmnat1 Lyophilized Standard 2 x 1 ng 100X Nmnat1 Biotinylated Detector Anitbody 1 x 120 uL 100X Nmnat1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL