ACKR4 ELISA Kit (Mouse)

A630091E18Rik; atypical chemokine receptor 4; C-C chemokine receptor type 11; CC chemokine receptor-like 1; C-C CKR-11; CCBP; CCBP2; CC-CKR-11; Ccr; CCR1; CCR11; CCR-11; Ccrl1; CCX CKR; CCX-CK; CCX-CKR; CCX-CKR1; chemokine (C-C motif) receptor-like 1; chemokine (C-C) receptor-like 1; chemokine receptor CCR11; Cmkbrl1; P; PPR1; VSH; VSHK1.

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CCL2, CCL8, CCL13, CCL19, CCL21 and CCL25. Chemokine-binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization. Plays an important role in controlling the migration of immune and cancer cells that express chemokine receptors CCR7 and CCR9, by reducing the availability of CCL19, CCL21, and CCL25 through internalization. Negatively regulates CXCR3-induced chemotaxis. Regulates T-cell development in the thymus and inhibits spontaneous autoimmunity.

Aviva Systems Biology Ackr4 ELISA Kit (Mouse) (OKWB00247) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ackr4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ackr4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ackr4 captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type Range Mean Serum (n=5) 86-99 92 EDTA Plasma (n=5) 95-102 100 Heparin Plasma (n=5) 89-98 95 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-99 92 Serum (n=5) 86-99 92 EDTA Plasma (n=5) 95-102 100 EDTA Plasma (n=5) 95-102 100 Heparin Plasma (n=5) 89-98 95 Heparin Plasma (n=5) 89-98 95

Component Amount Ackr4 Microplate 96 Wells (12 x 8 Well strips) Ackr4 Lyophilized Standard 2 x 10 ng 100X Ackr4 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Ackr4 Microplate 96 Wells (12 x 8 Well strips) Ackr4 Microplate 96 Wells (12 x 8 Well strips) Ackr4 Lyophilized Standard 2 x 10 ng Ackr4 Lyophilized Standard 2 x 10 ng 100X Ackr4 Biotinylated Detector Anitbody 1 x 120 uL 100X Ackr4 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL